Asian Elephant, Thi, is pregnant again, but keepers are worried for both her and her unborn calf. Male Clownfish Polo, is injured while fighting with the other males for the attention of dominant female, Rosie. A new male Giant Anteater arrives as a potential mate for Bliss and Giraffe, Meru, is on a mission.
Andean Spectacled bears, Lima, and her daughter, Madidi, aren't getting along. Blue Morpho butterflies, Macuna and Peanut, need to produce the next generation, but when they get drunk on fermented fruit, things are up in the air. After platonic mate, Diego, is moved to another zoo, Giant Otter, Icana, is introduced to a new mate.
Vets at Chester Zoo treat Asian elephant calf Indali for EEHV (elephant endotheliotropic herpes virus), the same virus that ended the lives of calves Aayu and Nandita in October of last year. The Cardinal fish are breeding and the Humboldt penguin colony welcomes 16 new members while penguins Almond and her mate Walnut go house-hunting.
Newcomer Safi, a rare bongo antelope from the mountains of Kenya, is giving enthusiastic breeding partner Moti the runaround. Very soon the keepers realise why - it seems she may be about to give birth earlier than expected. Sunda gharial crocodiles Frank and Frankie need to have their blood tested - but taking a sample from one of the world's largest and most dangerous reptiles is easier said than done - and George the giant rat needs to have surgery after an X-ray pinpoints a huge lump of hard faeces in his stomach.
Compilation of highlights from previous episodes. An orangutan has to be separated from his mother for the first time when she undergoes a CT scan to check for a life-threatening infection. The zoo's cardinal fish undergo a breeding programme, but one struggles to protect his offspring from his circling, cannibalistic neighbours.