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Yes

Megumi Hayashibara as Rei Ayanami / Yui Ikari (voice)
Minnie Driver as Brooke Shields (voice)
Bruce Timm as Booster Gold (voice)
Ikue Otani as Tib (voice)
Anji Iwata as 山田 / Yamada
Michaela Dietz as Tomas
David Tennant as Charles Darwin (voice)
Robert Clotworthy as CRT Spokesman (voice)
Diane Keaton as Daphne (voice)
Jon Stewart as Robot
Kathreen Khavari as Badyah
Seann William Scott as Crash (voice)
Saffron Henderson as Callisto
Emily Tunon as Lady Doppler
Russi Taylor as Widow Tweed (voice)
Aya Yamane as Misao Makimachi
Tabitha St. Germain as Miette Walla (voice)
Yuuichi Iguchi as Mitsuki Sarue
Maki Tsuruta as Retsuko's Mother
Shelby Young as Magician
Jason O'Mara as Batman / Bruce Wayne (voice)
Miriam Margolyes as Fly (voice)
Jordan Orr as (voice)
Junichi Suwabe as Saran Mithray

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