Sakurako gets pushed around a bit by her sisters Nadeshiko and Hanako whilst Himawari's little sister Kaede is a bit too inquisitive. Meanwhile, Chizuru decides to clean up Chitose's room whilst she is out, but has to take extreme measures to stop picturing her and Ayano together and drooling all over the place. Later, Himawari worries that she's gained weight and comes to Sakurako for some exercise advice. Elsewhere, Chinatsu talks with her older sister, Tomoko, who has a crush on Akari's older sister, Akane, who, at that moment, is doing perverted things with Akari's belongings whilst she is away.
生徒会次期副会長、大室櫻子とは私のことだー! ……ん? なんか一年前もそんなこと言ってたような……。
よし! 細かいことは気にしないぞ!
それより聞いてよ~。うちのねーちゃんと妹が私に厳しくてさ。これはきっと、私のほとばしるびぼうとちせいにしっとしてるに違いないね!
しかし、どこの家も姉とか妹ってこんななの? あっ、でも向日葵んとこの楓はすごくできた妹だっけ。姉とは違って! うん、姉とは違って!
楓、私の妹になんない? 向日葵が冷蔵庫の上から三段目の奥の豆腐の陰に隠してるプリンあげるから。
개성 넘치는 유루유리의 주요 등장인물들의 언니와 여동생이 대거 출현한다. 각각의 주요 등장인물들에 버금갈 만큼 독특한 개성으로 똘똘 뭉친 자매들을 제각기 다른 에피소드를 통해 소개하는데…
As irmãs de Yuru Yuri finalmente conseguem um tempinho na tela e elas são tão únicas quanto as meninas do Clube de Diversões.