Ryan's rest on the Amazon River boat has come to an end. It is now time to test of Ryan's resolve and put his survival skills to the test, but not before a quick flight over the Rio Negra River. After exploring the Amazon rainforest from the air Ryan meets up with Leo, a tree climbing and jungle survival expert. Leo takes Ryan 50m up in to the Amazon canopy as they climb "The Iron Tree" and then spend a night drinking jungle alcohol made from the Sacacura Mira tree bark. Ryan barely survives his night in the jungle, and his reward is a soul crushing muddy 900km journey along the most dangerous road in South America, known as the "Road of Ghosts" - The BR-319.