Pentatonix performed the opening theme; Tonight Show Superlatives; Tonight Show #hashtags: #WhyDidISayThat; Tonight Show Kid Theater (Tom Hanks); Pentatonix performed "Can't Sleep Love"
| Name | Type | Role | |
|---|---|---|---|
| Pentatonix | Guest Star | ||
| Kirstin Maldonado | Guest Star | ||
| Tom Hanks | Guest Star | ||
| Kevin Olusola | Guest Star | ||
| Jessica Chastain | Guest Star | ||
| Avi Kaplan | Guest Star | ||
| Scott Hoying | Guest Star | ||
| Mitch Grassi | Guest Star |