Mio still experiences power drains from her use of the true Reppuzan. Later, she scolds Yoshika for touching her sword--which reacted to her powers and nearly caused her to faint. Later, Mio and Minna receive orders from their superiors for a final attack on the Neuroi nest. They plan to defeat it by powering the Yamato with Neuroi technology. The mission's failure would mean the surrender of Romagna and the disbanding of the 501st Strike Witches. Mio becomes determined to complete the True Reppuzan, but Minna, aware that her sword has drained nearly all of her magical power, stops her--which Yoshika overhears. The next day, they begin the charge and Mio's Reppuzan fails to work, much to her despair. Meanwhile, the Yamato activates its Neuroi Dynamo, allowing it to fly and repair itself. But when the Neuroified Yamato hits the hive bow first, its Neuroi Dynamo stopped working, paralyzing its firing controls. With the mission deemed a failure, Mio, never giving up, charges towards the Yamato to attempt to restart the Dynamo with her remaining magic power.
坂本の魔法力が限界になりつつある中、ヴェネツィア上空にあるネウロイの巣への総攻撃作戦が開始されることになった。そしてミーナ率いる、第501統合戦闘航空団は連合軍の決戦兵器を護衛することを命じられたが…。
Mio todavía experimenta drenajes de energía de su uso de la Verdadera Reppuzan. Más tarde, descubre que reacciona Yoshika espada de Mio a sus poderes y casi la hace desmayarse. Más tarde, Mio y Minna reciben órdenes de sus superiores para un ataque final sobre el nido Neurol. Ellos planean derrotarlo alimentando el Yamato con la tecnología Neurol.
