Take a microtonal song and try to play it in 12edo (standard musical tuning in the West). The original was in a just intonation scale built using only harmonic overtones. In my opinion the biggest difference in this 12edo version is not how the melody is affected, but how there is now a subtle chorusing sound on the synth instruments because the inaccuracy of equal tempered tuning creates a rich beating within the overtones of overlapping notes.